Bioremediation of lead using spore surface displayed proteins

Lead is a toxic pollutant very harmful to human health since it accumulates in the body and affects the brain, liver, kidney, and bones. Fetuses can be exposed to lead during pregnancy, which can cause problems with learning later on in the baby’s life. The purpose of this project is to display a lead binding protein on bacterial spore surface for the bioremediation of lead from water. Spores from Bacillus subtilis are very robust and resistant to various harsh environments. Genetically fused to a spore surface protein, the displayed proteins demonstrate enhanced robustness and can be easily produced through sporulation without the need of further purification. PbrR is a regulatory protein that modulates the lead resistance in bacteria. In this project, we fused seven variants of PbrR to the spore surface protein CotC (CotC-PbrRs). The lead binding affinity and specificity of these PbrR variants on the spore surface will be characterized. The robustness of these spores with PbrR will be also evaluated using wastewater samples

Isospin in Reaction Dynamics. The Case of Dissipative Collisions at Fermi Energies

A key question in the physics of unstable nuclei is the knowledge of the $EOS$ for asymmetric nuclear matter ($ANM$) away from normal conditions. We recall that the symmetry energy at low densities has important effects on the neutron skin structure, while the knowledge in high densities region is crucial for supernovae dynamics and neutron star properties. The $only$ way to probe such region of the isovector $EOS$ in terrestrial laboratories is through very dissipative collisions of asymmetric (up to exotic) heavy ions from low to relativistic energies. A general introduction to the topic is firstly presented. We pass then to a detailed discussion on the $neck-fragmentation$ process as the main dissipative mechanism at the Fermi energies and to the related isospin dynamics. From Stochastic Mean Field simulations the isospin effects on all the phases of the reaction dynamics are thoroughly analysed, from the fast nucleon emission to the mid-rapidity fragment formation up to the dynamical fission of the $spectator$ residues. Simulations have been performed with an increasing stiffness of the symmetry term of the $EOS$. Some differences have been noticed, especially for the fragment charge asymmetry. New isospin effects have been revealed from the correlation of fragment asymmetry with dynamical quantities at the freeze-out time. A series of isospin sensitive observables to be further measured are finally listed.Comment: 16 pages, 6 figures, Contribution to the 5th Italy-Japan Symposium, Recent Achievements and Perspectives in Nuclear Physics, Naples Nov.3-7 2004, World Sci. in press. Latex in WorldSci/proc/styl

On the Analytic Wavelet Transform

An exact and general expression for the analytic wavelet transform of a real-valued signal is constructed, resolving the time-dependent effects of non-negligible amplitude and frequency modulation. The analytic signal is first locally represented as a modulated oscillation, demodulated by its own instantaneous frequency, and then Taylor-expanded at each point in time. The terms in this expansion, called the instantaneous modulation functions, are time-varying functions which quantify, at increasingly higher orders, the local departures of the signal from a uniform sinusoidal oscillation. Closed-form expressions for these functions are found in terms of Bell polynomials and derivatives of the signal's instantaneous frequency and bandwidth. The analytic wavelet transform is shown to depend upon the interaction between the signal's instantaneous modulation functions and frequency-domain derivatives of the wavelet, inducing a hierarchy of departures of the transform away from a perfect representation of the signal. The form of these deviation terms suggests a set of conditions for matching the wavelet properties to suit the variability of the signal, in which case our expressions simplify considerably. One may then quantify the time-varying bias associated with signal estimation via wavelet ridge analysis, and choose wavelets to minimize this bias

Analysis of Modulated Multivariate Oscillations

The concept of a common modulated oscillation spanning multiple time series is formalized, a method for the recovery of such a signal from potentially noisy observations is proposed, and the time-varying bias properties of the recovery method are derived. The method, an extension of wavelet ridge analysis to the multivariate case, identifies the common oscillation by seeking, at each point in time, a frequency for which a bandpassed version of the signal obtains a local maximum in power. The lowest-order bias is shown to involve a quantity, termed the instantaneous curvature, which measures the strength of local quadratic modulation of the signal after demodulation by the common oscillation frequency. The bias can be made to be small if the analysis filter, or wavelet, can be chosen such that the signal's instantaneous curvature changes little over the filter time scale. An application is presented to the detection of vortex motions in a set of freely-drifting oceanographic instruments tracking the ocean currents

The meaning of S-D dominance

The dominance of S and D pairs in the description of deformed nuclei is one of the facts that provided sustain to the Interacting Boson Approximation. In Ref.(J. Dukelsky and S. Pittel, Phys. Rev. Lett. 86, 4791, 2001.), using an exactly solvable model with a repulsive pairing interaction between bosons it has been shown that the ground state is described almost completely in terms of S and D bosons. In the present paper we study the excited states obtained within this exactly solvable hamiltonian and show that in order to obtain a rotational spectra all the other degrees of freedom are needed.Comment: Are S and D pairs enough to describe deformed nuclei

Generalized Morse Wavelets as a Superfamily of Analytic Wavelets

The generalized Morse wavelets are shown to constitute a superfamily that essentially encompasses all other commonly used analytic wavelets, subsuming eight apparently distinct types of analysis filters into a single common form. This superfamily of analytic wavelets provides a framework for systematically investigating wavelet suitability for various applications. In addition to a parameter controlling the time-domain duration or Fourier-domain bandwidth, the wavelet {\em shape} with fixed bandwidth may be modified by varying a second parameter, called $\gamma$. For integer values of $\gamma$, the most symmetric, most nearly Gaussian, and generally most time-frequency concentrated member of the superfamily is found to occur for $\gamma=3$. These wavelets, known as "Airy wavelets," capture the essential idea of popular Morlet wavelet, while avoiding its deficiencies. They may be recommended as an ideal starting point for general purpose use

Variation of the Diameter of the Sun as Measured by the Solar Disk Sextant (SDS)

The balloon-borne Solar Disk Sextant (SDS) experiment has measured the angular size of the Sun on seven occasions spanning the years 1992 to 2011. The solar half-diameter -- observed in a 100-nm wide passband centred at 615 nm -- is found to vary over that period by up to 200 mas, while the typical estimated uncertainty of each measure is 20 mas. The diameter variation is not in phase with the solar activity cycle; thus, the measured diameter variation cannot be explained as an observational artefact of surface activity. Other possible instrument-related explanations for the observed variation are considered but found unlikely, leading us to conclude that the variation is real. The SDS is described here in detail, as is the complete analysis procedure necessary to calibrate the instrument and allow comparison of diameter measures across decades.Comment: 41 pages; appendix and 2 figures added plus some changes in text based on referee's comments; to appear in MNRA

Solar radius and luminosity variations induced by the internal dynamo magnetic fields

Although the occurrence of solar irradiance variations induced by magnetic surface features (e.g., sunspots, faculae, magnetic network) is generally accepted, the existence of intrinsic luminosity changes due to the internal magnetic fields is still controversial. This additional contribution is expected to be accompanied by radius variations, and to be potentially significant for the climate of the Earth. We aim to constrain theoretically the radius and luminosity variations of the Sun that are due to the effect of the variable magnetic fields in its interior associated with the dynamo cycle. We have extended a one-dimensional stellar evolution code to include several effects of the magnetic fields on the interior structure. We investigate different magnetic configurations, based on both observational constraints and on the output of state-of-the-art mean field dynamo models. We explore both step-like and simply periodic time dependences of the magnetic field peak strength. We find that the luminosity and radius variations are in anti-phase and in phase, respectively, with the magnetic field strength. For peak magnetic field strengths of the order of tens of kilogauss, luminosity variations ranging between 10^{-6} and 10^{-3} (in modulus) and radius variations between 10^{-6} and 10^{-5} are obtained. Modest but significant radius variations (up to 10^{-5} in relative terms) are obtained for magnetic fields of realistic strength and geometry, providing a potentially observable signature of the intrinsic variations. Establishing their existence in addition to the accepted surface effects would have very important implications for the understanding of solar-induced long-term trends on climate.Comment: 18 pages, 7 figures; accepted for publication in Astronomische Nachrichte

Optimal control of a dengue epidemic model with vaccination

We present a SIR+ASI epidemic model to describe the interaction between human and dengue fever mosquito populations. A control strategy in the form of vaccination, to decrease the number of infected individuals, is used. An optimal control approach is applied in order to find the best way to fight the disease.Comment: This is a preprint of a paper accepted for presentation at ICNAAM 2011, Halkidiki, Greece, 19-25 September 2011, and to appear in AIP Conference Proceedings, volume 138

Modeling and Optimal Control Applied to a Vector Borne Disease

A model with six mutually-exclusive compartments related to Dengue disease is presented. In this model there are three vector control tools: insecticides (larvicide and adulticide) and mechanical control. The problem is studied using an Optimal Control (OC) approach. The human data for the model is based on the Cape Verde Dengue outbreak. Some control measures are simulated and their consequences analyzed